인플루엔자바이러스 M2 protein 및 neuraminidase (NA) 억제제에 대한 내성주 분포 및 특성 조사

초록

Purpose : To investigate the frequency of influenza virus resistance to M2 protein inhibitor(amantadine) and neuraminidase inhibitors(oseltamivir & zanamivir) in Korea during 2006-2007 season, we examined genetic and phenotypic analyses of influenza viruses isolated by Korean Influenza Surveillance Scheme(KISS). Methods : For genetic analysis of influenza viruses, we analyzed M2 gene(74 A/H1, 394 A/H3) and NA gene(79 A/H1, 529 A/H3, & 45 B isolates) by RT-PCR followed by pyrosequecing and conventional Sanger sequencing, respectively. Reduction of virus susceptibility to M2 inhibitor and NA inhibitors was observed by haemagglutination(HA) assay and fluorometric NA inhibition assay, respectively. Results : Among the 468 influenza A isolates, the rates of amantadine resistance of A/H1 and A/H3 were 81% and 57% in 2006-2007 season, respectively. Fifteen influenza A isolates containing both sensitive and resistant isolates identified by sequencing were correlated with phenotypes results of HA assay. 653 isolates(79 A/H1, 529 A/H3, and 45 B) and 336 isolates(60 A/H1, 244 A/H3, and 32 B) showed oseltamivir susceptibility and zanamivir susceptibility, respectively, by sequencing analysis and NA inhibition assay. Conclusions : The amantadine resistant influenza A/H3 prevailed during this season has decreased compared those of to last season, whereas the amantadine resistant influenza A/H1 has increased rapidly during this season. Although there was no oseltamivir resistant isolates in Korea, the surveillance of drug resistant viruses should be monitored carefully for the emergence of viral resistance.

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