Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology

  • Hong, JiYoung; 
  • Kang, Byunghak; 
  • Kim, Ahyoun; 
  • Hwang, Seoyoun; 
  • Lee, Sunwha; 
  • 외 4명
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초록

To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

키워드

POLYMERASE-CHAIN-REACTION; CEREBROSPINAL-FLUID SPECIMENS; CENTRAL-NERVOUS-SYSTEM; RAPID DETECTION; PATIENT-MANAGEMENT; ASEPTIC-MENINGITIS; DIAGNOSIS; ASSAY; INFECTIONS; UTILITY
제목
Enhanced Detection of Enteroviruses in Clinical Samples by Reverse Transcription-PCR Using Complementary Locked Primer Technology
저자
Hong, JiYoung; Kang, Byunghak; Kim, Ahyoun; Hwang, Seoyoun; Lee, Sunwha; Kim, Jonghyen; Lee, Hyun-Young; Kang, Sang-Hyeon; Cheon, Doo-Sung
DOI
10.1128/JCM.01790-09
발행일
2010-02
유형
Article
저널명
Journal of Clinical Microbiology
권
48
호
2
페이지
615 ~ 616