Attachment and Detection of His-tag Protein on the Surface of Chelating Polydiacetylene Liposomes

초록

Polydiacetylene (PDA) is a unique conjugated liposome that shows a significant colorimetric change in response to a variety of external environments such as temperature, pH and molecular recognition. In this study, we demonstrated that PDA liposome can be functionalized with Ni-NTA DOGS (1,2-dioleoyl-sn-glycero-3-{[N(5-amino-1-carboxypentyl) iminodiacetic acid] succinyl} (nickel salt) lipid to interact with His-tag protein, which could be analyzed qualitatively by colorimetric detection. The His-tag green fluorescent protein (GFP) extracted from the jellyfish, Aequorea Victoria, was expressed in bacteria and purified as a model protein. It was observed that Ni-NTA functionalized PDA liposome could specifically interact with the protein through chelation. Although further studies for sensitivity, specificity, and stability improvements should be necessary for clinical applications, it is believed that the results of Ni-nitrilotriacetic acid (NTA)/methoxy polyethylene glycol (MPEG)-10,12-pentacosadiynoic acid (PCDA)/PCDA liposome with His-tag GFP present a potential platform system for simple detection and effective delivery of proteins in various biotechnology fields.

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