Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a modified RNA system

초록

We generated human induced pluripotent stem cells (KSCBi002-B and KSCBi002-B-1) from the dermal fibroblasts of a donor using a modified RNA-based gene deliverymethod. According to GTG-banding analysis, the generated KSCBi002-B line has a cytogenetic abnormality (46, XY, t(1;4)(q21;q25)) that is distinct from that of the donor, whereas KSCBi002-B-1 has a normal karyotype (46, XY). These cell lines can be useful as a model for characterizing the hiPSCs generated by a non-viral and non-integrative system, or as a chromosomal balanced translocation model. These two cell lines are registered and available from the National Stem Cell Bank, Korea National Institute of Health. (C) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.