Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp

Kim, Junyoung; Jeon, Semi; Kim, Hyungjun; Park, Misun; Kim, Soobok; Kim, Seonghan

doi:10.1016/j.phrp.2012.04.004

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Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp

Kim, Junyoung;
Jeon, Semi;
Kim, Hyungjun;
Park, Misun;
Kim, Soobok;
... Kim, Seonghan

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초록

Two real-time polymerase chain reaction assays were developed to detect mutations in codons 83 and 87 in gyrA and in codons 80 and 91 in parC, the main sites that causes quinolone resistance in pathogenic Escherichia coli and Shigella spp. isolates. These assays can be employed as a useful method for controlling infections caused by quinolone-resistant E coli and Shigella isolates. © 2012.

키워드

Fluoroquinolone; GyrA; Multiplex real-time PCR; ParC

제목: Multiplex Real-Time Polymerase Chain Reaction-Based Method for the Rapid Detection of gyrA and parC Mutations in Quinolone-Resistant Escherichia coli and Shigella spp

저자: Kim, Junyoung; Jeon, Semi; Kim, Hyungjun; Park, Misun; Kim, Soobok; Kim, Seonghan

DOI: 10.1016/j.phrp.2012.04.004

발행일: 2012-06

유형: Article

저널명: Osong Public Health and Research Persptectives

권: 3

호: 2

페이지: 113 ~ 117

언어: ENG

출판사: Korean Disease Control and Prevention Agency

발행국가: 대한민국

분량: 5 페이지

ISSN: E 2233-6052
P 2210-9099