IDENTIFICATION OF TUMOR NECROSIS FACTOR SIGNALING-RELATED PROTEINS DURING EPSTEIN-BARR VIRUS-INDUCED B CELL TRANSFORMATION

초록

Epstein-Barr virus (EBV) infection in vitro transforms primary B cells into Continuously proliferating lymphoblastoid cell lines (LCLs) that have been widely used as a genomic resource For variety of immunological and genetic studies. However, the biochemical and biological,characteristics that distinguish LCLs from the B cells have not been thoroughly investigated. Our proteomic approach showed that EBV infection induced changes in the profiles of Minor necrosis factor (TNF) signaling-related proteins in LCLs including heat shock protein Family members TNF receptor-associated protein 1 (TRAP-1), heat shock 70-kDa protein 9 (HSPA9)) and superoxide dismutase 2 (SOD2). In addition, our literature co-occurrence study placed TNF at the center of a gene cluster network of differentially expressed proteins in LCLs. This study, suggested that deregulation of TNF signaling pathway Could contribute to the Cellular transformation and immortalization of the EBV-infected B cells.

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