Generation of integration-free induced pluripotent stem cell line (KSCBi024-A) from patients with down syndrome

초록

We established an integration-free human induced pluripotent stem cell (hiPSC) line, KSCBi024-A, from peripheral blood mononuclear cells (PBMCs) of a 13-year-old male with Down syndrome (trisomy 21). Cytogenetic analysis confirmed a 46,XY,i(21)(q10) karyotype, indicating an isochromosome 21q. Reprogramming was carried out using non-integrating episomal vectors, and the resulting hiPSCs exhibited typical pluripotent stem cell morphology and expressed core pluripotency markers (OCT4, NANOG, SOX2) at levels comparable to human embryonic stem cells. The line demonstrated trilineage differentiation potential in vitro and was free of mycoplasma contamination. KSCBi024-A represents a useful resource for modeling trisomy 21-associated cellular phenotypes and is available through the Korea National Stem Cell Bank.

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