원인 불명 호흡기 검체로부터 신종 호흡기바이러스 검출 및 특성 규명 - 휴먼 보카바이러스(bocavirus) 국내 분리주의 특성연구

초록

Purpose : Human bocavirus(HBoV) is a parvovirus that has been recently detected in patients with respiratory illness. We developed a sensitive, specific, and quantitative real-time PCR assay based on the TaqMan method for HBoV detection and quantification in respiratory specimens. Methods : Three individual real-time PCR assays were designed to amplify HBoV NS1, NP-1, and VP1 genes. For clinical evaluation, 506 nasal aspirates obtained from patients with acute respiratory tract infections during December 2006 through May 2007 were tested. Results : Each assay had a broad dynamic range(50 to 5x10⁷ copies of plasmid DNA) and high inter- and intraassay reproducibility. The detection limit of each assay was 10 genome copies per reaction, and no cross reactivity with other major respiratory viruses or bacteria was detected. Clinical evaluation revealed that 11(2.1%) of 506 patients diagnosed with upper respiratory tract infections, pneumonia, bronchitis, pharyngitis, or sinusitis had HBoV detected by all three assays, with viral loads ranging from 8.2x10⁴ to 8.1x10⁹ copies/ml of specimen. Conclusions : The three assays for HBoV diagnosis and quantification are highly sensitive, specific real-time tools for the reliable epidemiological and pathogenetic study of HBoV infection.

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